Morphology observation of dipalmitoyl phosphatidyl choline (DPPC) monolayer on water surface by dropping method

Yoshida, Daisuke and Yokoyama, Takashi and Shimoaki, Takatsugu and Tomita, Takashi and Yoshida, Tadayoshi and Yamamoto, Yasushi and Taga, Keijiro and Sumino, Ayumi and Dewa, Takehisa and Nango, Mamoru and Yamamoto, Masato and Sheravani, Zameer (2013) Morphology observation of dipalmitoyl phosphatidyl choline (DPPC) monolayer on water surface by dropping method. Journal of Biophysical Chemistry, 04 (04). pp. 115-121. ISSN 2153-036X

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Abstract

Preparation of DPPC lipid monolayer in water trough has been done by dropping method and compared with compression method. Monolayer was studied by surface pressure isotherm, fluorescence microscopy, Brewster angle microscopy, and infrared external reflection spectroscopy. Results of these measurements showed that dropping method gave better results compared to compression method. In dropping method, transition from liquid expanded state to liquid condensed is gradual compared to sharp one in compressed method. During monolayer formation, adjustment and interaction between hydrophilic part of lipid and water and among hydrophobic part of lipid molecule are slow, stable, and more natural as worked out from surface area versus pressure isotherm. At a given molecular area, surface pressure is less compared to compression method thus monolayer is in more fluidic state in dropping method than compression method. The observation was supported by all techniques described above.

Item Type: Article
Subjects: STM Library > Chemical Science
Depositing User: Managing Editor
Date Deposited: 31 Jan 2023 05:47
Last Modified: 05 Jun 2024 09:33
URI: http://open.journal4submit.com/id/eprint/888

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