Expression of Estrogen Receptor α by Decidual Macrophages in Preeclampsia

Vishnyakova, Polina and Poltavets, Anastasiya and Nikitina, Maria and Midiber, Konstantin and Mikhaleva, Liudmila and Muminova, Kamilla and Potapova, Alena and Khodzhaeva, Zulfiya and Pyregov, Alexey and Elchaninov, Andrey and Fatkhudinov, Timur and Sukhikh, Gennady (2021) Expression of Estrogen Receptor α by Decidual Macrophages in Preeclampsia. Biomedicines, 9 (2). p. 191. ISSN 2227-9059

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Abstract

Preeclampsia is a gestation-associated hypertensive syndrome that threatens the life and health of the mother and the child. The condition is presumably caused by systemic failure with a strong involvement of innate immunity. In particular, it has been associated with flexible phenotypes of macrophages, which depend on the molecules circulating in the blood and tissue fluid, such as cytokines and hormones. This study aimed at a comparative evaluation of pro-inflammatory (TNFα) and anti-inflammatory (CD206, MMP9, HGF) markers, as well as the levels of estrogen receptor α, expressed by decidual macrophages in normal pregnancy and in patients with early- and late-onset preeclampsia. The tissue samples of decidua basalis were examined by immunohistochemistry and Western blotting. Isolation of decidual macrophages and their characterization were performed using cultural methods, flow cytometry and real-time PCR. Over 50% of the isolated decidual macrophages were positive for the pan-macrophage marker CD68. In the early-onset preeclampsia group, the levels of estrogen receptor α in decidua were significantly decreased. Furthermore, significantly decreased levels of HGF and CD206 were observed in both preeclampsia groups compared with the control group. The observed downregulation of estrogen receptor α, HGF and CD206 may contribute to the balance of pro- and anti-inflammatory macrophages and thereby to pathogenesis of preeclampsia.

Item Type: Article
Subjects: STM Library > Medical Science
Depositing User: Managing Editor
Date Deposited: 22 Feb 2023 05:59
Last Modified: 12 Mar 2024 04:08
URI: http://open.journal4submit.com/id/eprint/817

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