Agrobacterium-mediated Genetic Transformation of Groundnut (Arachis hypogaea L.) using Epicotyl Explants

Eapen, Yogranjan, S. and Tiwari, S. and Tripathi, M. K. (2006) Agrobacterium-mediated Genetic Transformation of Groundnut (Arachis hypogaea L.) using Epicotyl Explants. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 7 (1-2). pp. 73-76.

Full text not available from this repository.

Abstract

Transformation of groundnut (Arachis hypogaea L.) was achieved using Agrobacterium tumefaciens strain LBA4404 harboring binary vectors pHS102 (containing uidA, nptII and Cry1Ac genes) pBI121 (containing uidA (GUS) and nptII genes) pBinAR (possesses a fusion chimeric gene Cry1Ac-F gene). The transformation frequency varied from 4% (with pHS102) to 12% (with pBI121). A strong GUS expression was detected in the putative transformed shoots by the histochemical assay. Transformation was confirmed by the presence of 0.70 kb amplified fragments for nptII and 1.40 kb for uidA genes by PCR analysis. Shoot differentiation via callusing was attained on MS medium supplemented with 2 mg.l-1 BA, 0.1 mg.l-1 IAA, and 150 mg.l-1 adenine sulphate, whereas direct shoot differentiation was obtained on medium containing 0.1 mg.l-1 TDZ.

Item Type: Article
Subjects: STM Library > Biological Science
Depositing User: Managing Editor
Date Deposited: 08 Jan 2024 05:41
Last Modified: 08 Jan 2024 05:41
URI: http://open.journal4submit.com/id/eprint/3456

Actions (login required)

View Item
View Item