TLP Sequenced and Studied for Its Functions with Targets Diagnostic and Therapeutic: Recent Perspective

From the first analysis of immuneprecipitation followed by Western Blotting (WB) Corin and TLP seem
to precipitate at the same height (approximately 50 KDa) and are recognized by the same antibodies.
In parallel the tests of immunoprecipitation by the use of cell extracts derived from lung cancer cells
A549 and NCI-H23 are improved with the aim to be able of obtaining a precipitate containing only the
TLP. In fact the partial aminoacid sequence of TLP showes a high homology with the sequence of
human Corin (only one aminoacid is different) and is present in lung cancer under different isoforms. It
is known that human Corin is expressed mostly outside the cells and the protein extract derived from
the extracellular medium and from the cells transfected with the plasmid, which overexpresses Corin,
showes many more bands analyzed on SDS-PAGE that are equivalent to the bands (about 50-100
KDa) observed in the WB analyzed with anti-TLP.