Prospection of Endophyte Microorganisms from Bauhinia monandra Leaves with Mainly Identification of Actinobacteria

The present work aimed the prospection of microorganisms from Bauhinia monandra leaves, with the
purpose to identify endophytics to obtain strains with possible biotechnological applications. B.
monandra leaves, disinfected with hypochlorite solution, were macerated in phosphate buffered saline
and seeded in ten culture media containing antibacterial or antifungal agents. The endophytic
filamentous fungus strains detected belonged to the genera Penicillium, Curvullaria and Aspergillus.
Non-filamentous endophyte bacteria were grouped in the genera Bacillus, Burkholderia, Enterobacter
and strains of endophytic Actinobacteria were classified as Streptomyces and Nocardiopsis. The
isolation of endophytic microorganisms with nine culture media revealed better bacteria development
with L-arginine agar; inorganic salt starch agar and potato dextrose agar were superior to
Actinobacteria and fungus strains, respectively. The present study revealed the predominance of the
genus Penicillium in leaves of B. monandra. This work introduces the first data of identification from
endophyte Actinobacteria in the leaves of B. monandra.