Methodology of Micropropagation of Elite Genotype in Lotus (Nelumbo nucifera) Genotype Lakshmi

The lotus (Nelumbo nucifera Gaertn.) is an aquatic plant grown extensively throughout Asia, mostly for commercial and ornamental purposes. It is mostly propagated via rhizomes. A potent and reproducible plant regeneration system for lotus has been developed in this work employing rhizomes, shoot tips from the mother plant, and mature and immature embryos collected from eighteen and twenty-six-day-old aseptically fertilized seed. Shoot induction, number of shoots, and length of roots were induced on Murashige and Skoog's (MS) basal medium supplemented with various concentrations of 6-Benzylaminopurine (BAP) mgL-1 (0.5, 1, 2, 3, 4). The response of the explant varied with different BAP concentrations. The most favorable outcomes were achieved using MS medium treated with 0.5 mgL-1 6-Benzylaminopurine (T2).