Evaluation of Cysteine Metabolism in the Rat Liver and Kidney Following Intravenous Cocaine Administration and Abstinence

Kowalczyk-Pachel, Danuta and Iciek, Małgorzata and Bilska-Wilkosz, Anna and Górny, Magdalena and Jastrzębska, Joanna and Kamińska, Kinga and Dudzik, Paulina and Filip, Małgorzata and Lorenc-Koci, Elżbieta (2021) Evaluation of Cysteine Metabolism in the Rat Liver and Kidney Following Intravenous Cocaine Administration and Abstinence. Antioxidants, 10 (1). p. 74. ISSN 2076-3921

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Abstract

Many toxic effects of cocaine are attributed to reactive oxygen species (ROS) generated during its metabolism. Recently, it has been suggested that the biological action of ROS is often confused with endogenously generated reactive sulfur species (RSS). The aim of this study was to evaluate the impact of cocaine on thiols and RSS in the rat liver and kidney in the drug self-administration (SA) paradigm and the cocaine yoked delivery model (YC) followed by drug abstinence with extinction training. The level of thiols as well as RSS formed during anaerobic metabolism of cysteine and sulfate were assayed. In addition, the activity of enzymes involved in RSS formation and glutathione metabolism were determined. In the liver, following direct cocaine administration (SA and YC), the RSS levels decreased, while in the kidneys, cocaine increased the RSS contents in both groups. These changes were maintained in these tissues during drug abstinence. The level of sulfates was changed by cocaine only in the liver. In the kidney, cocaine shifted cysteine metabolism towards an anaerobic pathway. Our study demonstrates for the first time the changes in cysteine metabolism and thiol levels in the liver and kidney of rats after cocaine self-administration and abstinence.

Item Type: Article
Subjects: STM Library > Agricultural and Food Science
Depositing User: Managing Editor
Date Deposited: 01 Jul 2023 06:54
Last Modified: 17 Oct 2023 05:07
URI: http://open.journal4submit.com/id/eprint/2438

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