Agrobacterium-mediated transient transformation of Pentalinon andrieuxii Müll. Arg.

Yam-Puc, Alejandro and Avilés-Berzunza, Elidé and Chan-Bacab, Manuel J. and Peña-Rodríguez, Luis Manuel and Godoy-Hernández, Gregorio (2012) Agrobacterium-mediated transient transformation of Pentalinon andrieuxii Müll. Arg. Advances in Bioscience and Biotechnology, 03 (03). pp. 256-258. ISSN 2156-8456

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Official URL: https://doi.org/10.4236/abb.2012.33035

Abstract

Sections of hypocotyls, roots and leaves from Pentalinon andrieuxii plantlets were transiently transformed with Agrobacterium tumefaciens LBA4404 bearing the binary plasmid pCAMBIA2301 with an interrupted β-Glucuronidase (GUS) gene. Histochemical GUS assays showed transient gene expression in all infected tissues, being older roots those which displayed the most intense GUS staining. To our knowledge, this is the first report of Pentalinon andrieuxii susceptibility to Agrobacterium tumefaciens-mediated genetic transformation.

Item Type:	Article
Subjects:	STM Library > Biological Science
Depositing User:	Managing Editor
Date Deposited:	22 Mar 2023 05:25
Last Modified:	29 Jan 2024 05:59
URI:	http://open.journal4submit.com/id/eprint/1691

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